Table 2 Gradient programme for the pre-defined method using a 150 mm × 2.1 mm column with 1.8 μm particles, with a flowrate of 0.4 ml/min.
Time | % A | % B |
|---|---|---|
0 | 87 | 13 |
0.5 | 87 | 13 |
10.0 | 50 | 50 |
10.75 | 5 | 95 |
12.25 | 5 | 95 |
12.5 | 87 | 13 |
15.0 | 87 | 13 |
Time | % A | % B |
|---|---|---|
0 | 87 | 13 |
0.5 | 87 | 13 |
10.0 | 50 | 50 |
10.75 | 5 | 95 |
12.25 | 5 | 95 |
12.5 | 87 | 13 |
15.0 | 87 | 13 |