Fig. 4
From: Proteomic profiling dataset of chemical perturbations in multiple biological backgrounds
Use case illustrations for GCP and P100 data query. (a) Connectivity query of chromatin signatures of EZH2 wild-type (GA-10) and EZH2 mutant (NB4) cell lines from the Cancer Cell Line Encyclopedia (CCLE)54. This query illustrates how the library can be used to validate a presumptive gain-of-function mutation. Results are sorted from bottom to top ranks for the NB4 line (bottom 5% shown here) and identify EZH2 inhibitors (CPI-169, EPZ-005687, and GSK-126, highlighted in blue) as the most anti-connected hits. (b) Query results and connectivity matrix of two gamma secretase inhibitors, BMS-906024 and Semagacestat, in NPCs and astrocytes. For both drugs, the first ten rows correspond to the top ten most connected drugs to astrocytes, and the bottom ten rows to the top ten most connected drugs to NPCs. This query illustrates how the library can provide insight to a compound’s mechanism of action in differentiated cell types.
