Fig. 7
From: Systematic phenotyping and characterization of the 5xFAD mouse model of Alzheimer’s disease
Immunostaining of lysosomes. (a,b) Representative stitched brain hemispheres of WT and 5xFAD shown with LAMP1/Thio-S staining at 4 and 18 months, and 4-, 8- and 18-months timepoints, respectively. (c,d,i,j) Quantification of Thio-S in cortex and hippocampus. (e,f,k,l) LAMP1 immunostaining for lysosomes reveals age-related changes of 5xFAD mice in percent area of the cortex and hippocampus covered by LAMP1. (g,h). In quantifying the ratio of LAMP1/Thio-S coverage, there was an age-related decrease, but no sex-related changes in the cortex. (m,n) A ratio of the percent area coverages of LAMP1 and Thio-S reveals age-related changes in the hippocampus of 5xFAD mice and no sex-related changes.
