Fig. 1 | Scientific Data

Fig. 1

From: Short-read and long-read RNA sequencing of mouse hematopoietic stem cells at bulk and single-cell levels

Fig. 1

Sample collection and workflow. (a) Sample collection and sequencing methods. Bone marrow cells (BMC) were isolated, and lineage negative (LIN−) cells were enriched using magnetic beads. Long-term (LT) and short-term (ST) hematopoietic stem cells (HSC) and multipotent progenitors (MPP) were sorted according to their surface makers: LT-HSC (Sca-1+c-Kit+CD34− CD135−), ST-HSC (Sca-1+ c-Kit+CD34+ CD135−) and MPP (Sca-1+ c-Kit+CD34+ CD135+). Single-cell or 100 cells (bulk P100) were sorted for library construction following the Smart-seq2 protocol. The cDNA libraries were used for short-read (Illumina Hiseq) or long-read (Nanopore or PacBio) sequencing. (b) Gating strategy for cell sorting. The main population was gated via FSC-A (forward scatter) and SSC-A (side scatter), and single cells were gated via FSC-A and FSC-H. FSC, forward scatter; SSC, side scatter; A, area; H, height.

Back to article page