Fig. 4 | Scientific Data

Fig. 4

From: Short-read and long-read RNA sequencing of mouse hematopoietic stem cells at bulk and single-cell levels

Fig. 4

Quality check of bulk long-read sequencing. (a) Boxplots showing the length of reads per sample for nanopore (left) or PacBio (right) sequencing. LT-HSC (red), ST-HSC (green) and MPP (blue). (b) Violin plots showing read quality per sample for nanopore (left) or PacBio (right) sequencing. The white dots represent the means and the dashed line indicates the quality value of 7. (c) Precision of assembly accuracy at exon and transcript levels of Stingtie2 without a reference. (d) Precision of assembly accuracy at exon and novel exons levels of Stingtie2 with a reference. (e) Correlation coefficients of gene expression among the three sequencing methods. (f) Principal Component Analysis (PCA) of all samples from bulk Illumina, PacBio and Nanopore sequencing methods after batch correction.

Back to article page