Fig. 6
Alternative splicing isoforms identified using the long-read sequencing and quantified using the short-read sequencing. Sashimi plots showing long-reads mapped to c-Myc (a) or Gata2 (b) in LT-HSC (red), ST-HSC (green) and MPP (blue) by Nanopore or PacBio sequencing. The identified nearly full-length transcripts (upper panel) and annotated transcripts in Ensembl (lower panel) for c-Myc (c) and Gata2 (d) were shown. Sashimi plots showing Illumina generated short reads that are mapped to the indicated (shaded by grey) region for c-Myc (e) or Gata2 (f ). (g) Sashimi plots of Mpl gene showing the decreased PSI of an alternative splicing event in LT-HSC (red), ST-HSC (green) and MPP (blue) by summing up the reads from all the single cells of each type. The percentage of spliced-in (PSI) for the indicated regions was calculated for each cell type. (h) Heatmap illustrates that the heterogeneity of alternative splicing of Mpl among single cells within each cell type. (i,j) Violin plots showing the normalized splicing junction counts of the short junction at chr4:118455281–118455644:- (i) and the long junction at chr4:118455281–118455668:- (j) in LT-HSC (red), ST-HSC (green) and MPP (blue).
