Online-only Table 3 Batch information and quality control for bulk long-read RNA-sequencing samples.

From: Short-read and long-read RNA sequencing of mouse hematopoietic stem cells at bulk and single-cell levels

GEO Accession ID

Sample Name in GEO

cDNA preparation batch

No. of P100 samples mixed

Qubit concentration (ng/ul)

cDNA peak (bp)

Sequencing methods

Sample ID

Run

Cell type

Stage

Number Of Reads

Number Of Bases

Median Read Length

Mean Read Length

N50

Mean Quality

Median Quality

Mapped Reads (%)

Reads >Q7:

SRR13861890

P_8W_LT-HSC

20181213_batch1, 20181219_batch2, 20181227_batch3

16

133

1323

PacBio

8-6

P04DY19171604-1_r64031_20191206_091315_4_G02

LT-HSC

8W

239366

238119916

763

994.8

1541

47.2

37.3

88.52

239366 (100.0%) 238.1 Mb

SRR13861889

P_8 W_ST-HSC

20181213_batch1, 20181219_batch2, 20181227_batch3

14

149

1314

PacBio

8-7

P04DY19171605-1_r64031_20191206_091315_4_G02

ST-HSC

8W

245745

226569997

696

922

1406

47.4

37.4

88.13

245745 (100.0%) 226.6 Mb

SRR13862062

P_8 W_MPP

20181213_batch1, 20181219_batch2, 20181227_batch3

14

122

1324

PacBio

8-8

P04DY19171606-1_r64031_20191206_091315_4_G02

MPP

8W

250940

230891305

695

920.1

1398

48.1

37.7

88.98

250940 (100.0%) 230.9 Mb

SRR13861859

N_8 W_LT-HSC_1

20181219_batch1

1

19.5

1683

Nanopore

8-L1

V959-0101

LT-HSC

8W

6356335

7343750072

932

1155.3

1365

10.8

10.9

99.74

6355843 (100.0%) 7343.5 Mb

SRR13862101

N_8 W_LT-HSC_2

20181219_batch2

1

17.1

1859

Nanopore

8-L2

V959-S01-0101

LT-HSC

8W

2371682

2530358055

864

1066.9

1232

10.7

10.9

99.45

2303881 (97.1%) 2462.3 Mb

SRR13862051

N_8 W_ST-HSC_1

20181219_batch1

2

13.75

1820

Nanopore

8-S1

V959-S01-0102

ST-HSC

8W

3758835

3964994999

850

1054.8

1215

10.6

10.7

99.37

3625553 (96.5%) 3834.3 Mb

SRR13861905

N_8 W_ST-HSC_2

20181227_batch2

2

9.13

1380

Nanopore

8-S2

V959-S01-0103

ST-HSC

8W

2037766

1985569474

809

974.4

1081

10.6

10.7

99.2

1968031 (96.6%) 1921.3 Mb

SRR13861894

N_8 W_MPP_1

20181219_batch1

1

16

1832

Nanopore

8-M1

V959-S01-0104

MPP

8W

3685213

3561785473

783

966.5

1068

10.6

10.8

99.47

3570384 (96.9%) 3457.0 Mb

SRR13862019

N_8 W_MPP_3

20181227_batch2

1

12.5

1367

Nanopore

8-M3

V959-S01-0105

MPP

8W

2140511

1982333637

772

926.1

1006

9.9

9.9

99.2

2016327 (94.2%) 1873.6 Mb

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