Fig. 6
From: ProMetIS, deep phenotyping of mouse models by combined proteomics and metabolomics analysis
Quality control of the LC-MS proteomics analytical sequences in the liver (top) and plasma (bottom). Left: An internal standard consisting of 11 iRT peptides (iRT kit; Biognosys) was spiked in all samples. Retention times of the 11 spiked in peptides from the iRT kit (Biognosys) are plotted in the chronological order of data acquisition. Mean standard deviation across all liver (respectively, plasma) samples injections are 27 s (respectively, 13.5 s). The sequences of the peptides are: P1: LGGNEQVTR, P2: YILAGVENSK, P3: GTFIIDPGGVIR, P4: GTFIIDPAAVIR, P5: GAGSSEPVTGLDAK, P6: TPVISGGPYEYR, P7: VEATFGVDESNAK, P8: TPVITGAPYEYR, P9: DGLDAASYYAPVR, P10: ADVTPADFSEWSK, P11: LFLQFGAQGSPFLK. Right: CVs of the raw protein intensities per sample type.
