Fig. 1 | Scientific Data

Fig. 1

From: Mass cytometric and transcriptomic profiling of epithelial-mesenchymal transitions in human mammary cell lines

Fig. 1

Induction of EMT in human mammary epithelial cell lines. (a) Experimental workflow. (b) Gating to select live cells. (c) E-Cadherin and Vimentin expression in HMLEs. Gating to select populations with E1-, E2-, EM-, or M-phenotype. (d) Percentages of HMLEs per gate and time point as in (c). (e) Phase contrast images of HMLEs. (f) E-Cadherin and Vimentin expression in MCF10As. (g) Percentage of MCF10As cells per gate and time point as in (f). (h) Phase contrast images of MCF10As. (i) E-Cadherin and Vimentin expression in HTERs. (j) Percentage of HTERs per gate and time point as in (i). (k) Phase contrast images of HTERs. (l) E-Cadherin and Vimentin expression in HSERs. (m) Percentage of HSERs per gate and time point as in (l). (n) Phase contrast images of HSERs. (o) E-Cadherin and Vimentin expression in HMLEs. (p) Percentage of HMLEs per gate and time point as in (o). (q) Phase contrast images of HMLEs. Scale bar = 10 µm. E1 = epithelial 1, E2 = epithelial 2, EM = hybrid epithelial-mesenchymal, M = mesenchymal.

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