Fig. 2

Transcriptomic profiling of EMT-undergoing mammary epithelial cells. (a) Gating to select populations of interest of HTERs for RNA sequencing. (b) Number of RNA sequencing reads assigned to genes per sample. (c) Average base quality (upper panel) and GC content (lower panel) for all samples. (d) Multidimensional scaling plot showing the first two dimensions. (e–g) Volcano plots showing the indicated differential gene expression analyses. Highlighted in red are genes with an adjusted p-value below 0.05. logFC = log2 fold change, E = epithelial, EM = hybrid epithelial-mesenchymal, M = mesenchymal.