Fig. 4 | Scientific Data

Fig. 4

From: Mass cytometric and transcriptomic profiling of epithelial-mesenchymal transitions in human mammary cell lines

Fig. 4

Multiplex mass cytometry profiling of EMT phenotypes. (a) Histogram overlays showing the antibody panel performance. (b) Gating to select live cells. (c) UMAPs showing TGFβ1-treated and control MCF10A and HMLE cells colored by biological replicates. (d–g) UMAPs showing the indicated cell lines colored either by day and treatment (left) or by marker expression using z score levels (right). For all UMAPs, 1,000 cells per condition and replicate were used. (h) Heatmap showing marker expression (columns) using z score levels for each cluster or cell line (rows). PhenoGraph clustering was applied per EMT cell line. Z scores were calculated on arcsinh-transformed data and per marker. Hierarchical clustering was performed using Euclidean distance and ward.D2 linkage. The blue rectangle highlights that cluster HSER_2 is phenotypically similar to Hs578T cells and fibroblasts. The bar plot on the right shows the frequency per cluster of cells of the respective day and treatment.

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