Table 2 List of filters and dichroic mirrors for each dye used in miFISH.
From: Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH
Dye | Excitation filter | Dichroic mirror | Emission filter |
|---|---|---|---|
Hoechst 33342 | 390/22 (Lumencor) | 440/40 (custom-made Polychroic 1 from Chroma) | FF01-447/60–25 (Semrock) |
AF488 | FF01-494/20–25 (Semrock) | FF506-Di 03-25x36 (Semrock) | FF01-527/20–25 (Semrock) |
AT542 | FF01-534/20-25 (Semrock) | 570/20 (custom-made Polychroic 1 from Chroma) | FF01-567/15-25 (Semrock) |
AF594 | FF01-586/20-25x5 (Semrock) | 630/27 (custom-made Polychroic 2 from Chroma) | FF01-628/32-25 (Semrock) |
AT647N | 631/28 (Lumencor) | 675/29 (custom-made Polychroic 1 from Chroma) | FF01-676/29-25 (Semrock) |
AF700 | ZET690/10x (Chroma) | 622/35 (custom-made Polychroic 2 from Chroma) | ET740/40x (Chroma) |
AF790 | 780 nm MaxLine laser clean-up filter (Semrock) | 808/35 (custom-made Polychroic 1 from Chroma) | FF01-832/37-25 (Semrock) |
