Online-only Table 1 Sample and sequencing information.
Stage | Sample ID | cDNA peak (bp) | Short-read data | Long-read data | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Clean reads (Million) | GC (%) | Q30 (%) | TIN (median) | Uniquely mapped reads (%) | Average mapped length (bp) | Mean read length (bp) | Number of reads (Million) | >Q7 | |||
E15.5 | E15.5_1 | 1282 | 19.3 | 43 | 90.7 | 50.6 | 86.8 | 295.37 | 754 | 2.3 | 98.10% |
E15.5_2 | 1310 | 21.6 | 44 | 90.7 | 51.8 | 88.46 | 294.78 | ||||
E15.5_3 | 1399 | 18 | 45 | 90.5 | 51.8 | 89.67 | 295.15 | ||||
E17.5 | E17.5_1 | 1393 | 28.5 | 42 | 91.2 | 45.4 | 86.41 | 295.46 | 845.8 | 2.8 | 98.00% |
E17.5_2 | 1384 | 26.3 | 42 | 90.9 | 45.9 | 86.08 | 295.46 | ||||
E17.5_3 | 1378 | 17.7 | 42.5 | 88.4 | 50.8 | 83.47 | 293.53 | ||||
E17.5_4 | 1388 | 15.7 | 42 | 90.8 | 45.5 | 83.81 | 295.53 | ||||
E17.5_5 | 1342 | 25 | 44 | 90.8 | 49 | 86.57 | 295.08 | ||||
E17.5_6 | 1316 | 22.9 | 42.5 | 92.9 | 49.4 | 84.36 | 295.56 | ||||
P1.5 | P1.5_1 | 1299 | 14.5 | 41 | 91.3 | 36 | 82.34 | 294.51 | 747 | 2.2 | 98.50% |
P1.5_2 | 1358 | 13.9 | 41 | 91.2 | 34.9 | 82.76 | 294.51 | ||||
P1.5_3 | 1402 | 33.2 | 42 | 92.4 | 41.7 | 86.2 | 295.54 | ||||
P1.5_4 | 1334 | 19.2 | 43 | 89.6 | 48.2 | 82.43 | 293.55 | ||||
P8 | P8_1 | 1311 | 44.7 | 42 | 94.8 | 32.2 | 90.02 | 298.13 | 782.6 | 2.7 | 98.40% |
P8_2 | 1309 | 45.6 | 40 | 95.4 | 25 | 86.61 | 298.3 | ||||
P8_3 | 1294 | 45.1 | 40 | 95.5 | 26.5 | 86.04 | 298.35 | ||||
P8_4 | 1307 | 44.3 | 42 | 95 | 33.2 | 91.77 | 298.29 | ||||
P60 | P60_1 | 1833 | 31.5 | 44 | 90.8 | 51.1 | 89.7 | 295.36 | 856.7 | 2.4 | 98.50% |
P60_2 | 1817 | 26.3 | 44 | 91.4 | 53.1 | 89.59 | 296.78 | ||||
P60_3 | 1394 | 14.6 | 43 | 86.6 | 55.3 | 84.71 | 291.35 |