Fig. 2

Quality assessment of the rabbit eye spectral assay library. (a) Retention time (RT) fit of +2 and +3 charge states of the sample peptides in the spectral library. (b) Number of observed b- and y- ion fragments in the spectral library. (c) Distribution of fragment ions by observed fragment charge states in the spectral library. (d) Distribution of precursors by the number of fragment ions per precursor. (e) Distribution of precursors by precursor m/z values across the acquired mass range in the assay library. (f) Distribution of precursors by precursor charge states. (g) Distribution of the observed peptides by peptide length in the assay library. (h) Distribution of proteins by the number of peptides per protein in the spectral library. (i) Distribution of precursor CCS values across the acquired mass range in the spectral library color-coded by precursor charge state.