Fig. 2
From: Proteomic characterisation of triple negative breast cancer cells following CDK4/6 inhibition
Validation of senescence-like morphology and immunofluorescence staining of key senescence biomarkers. Treatment of MDA-MB-231 cells with 1uM PD0332991 (known as Palbociclib) for 7 days or with an equivalent dose of DMSO as a control. (a) Immunofluorescence staining for Ki67. (b) Immunofluorescence staining for pRb (807/811). (c) Immunofluorescence staining for LMNB1. In all panels actin, a-Tubulin and Hoescht (nucleus) staining is used to image morphological features. All images were taken using the Opera high-throughput microscope (PerkinElmer).
