Table 2 Primary, secondary antibodies and dyes for immunofluorescence staining.
From: Proteomic characterisation of triple negative breast cancer cells following CDK4/6 inhibition
Antibodies/Dyes | Species | Dilution | Manufacturer |
|---|---|---|---|
pRb(807/811) | Rabbit | 1:1000 | Cell signaling (Cat 8516S) |
KI67 | Rabbit | 1:50 | Abcam (#16667) |
LMNB1 | Rabbit | 1:1000 | Abcam (#16048) |
Tubulin Alpha | Rat | 1:1000 | BioRad (Ref MCA78G) |
Alexa Fluor 488 Phalloidin | — | 1:1000 | Invitrogen (Cat A-12379) |
Alexa Fluor 568 Phalloidin | — | 1:1000 | Invitrogen (Cat A-12380) |
Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 | Rat | 1:1000 | Invitrogen (Cat A-11077) |
F(ab’)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Alexa Fluor 647 | Rabbit | 1:1000 | Invitrogen (Cat A-21246, Lot 2147629) |
Goat anti-Rabbit IgG (H+L) Secondary antibody Highly Cross-Adsorbed, Alexa Fluor 488 | Rabbit | 1:1000 | Invitrogen (Cat A-11034) |
Goat anti-rat IgG (H+L) Secondary antibody Cross-Adsorbed, Alexa Fluor 647 | Rat | 1:1000 | Invitrogen (Cat A-21247) |
Hoescht 33258 | — | 1:1000 | Invitrogen (Cat H3569) |
