Table 3 Quality data of RNA-sequencing and bioinformatics processing.

Sample Name	Group	RNA extract. method	RNA conc. (ng/µL)	RIN	Pre-alignment			Post-alignment
Sample Name	Group	RNA extract. method	RNA conc. (ng/µL)	RIN	Total reads	Avg. read length	Avg. quality*	Total reads	Aligned (%)	Avg. length	Avg. quality*
ScAAK7	Static	RNeasy Minikits	1.2	9.1	25994148	42.55	33.60	25851954	95.38	42.45	33.83
ScAAK8	SMG		1.2	9.1	22585197	42.54	33.65	22464022	95.80	42.44	33.87
ScAAK9	Static		2.2	8.6	23676028	42.54	33.65	23550183	96.06	42.44	33.87
ScAAK10	SMG		2.1	8.7	20942143	42.54	33.52	20829886	95.89	42.43	33.74
ScACS1.1	Static	PuroSPIN Total DNA Purification KIT	44	10	21673085	42.50	34.08	21649968	96.20	42.40	34.27
ScACS1.2	SMG		83	9.3	26393415	42.52	34.08	26363008	95.26	42.41	34.32
ScACS2.1	Static		93	9.6	24756374	42.50	34.03	24728910	96.41	42.39	34.23
ScACS2.2	SMG		68	9.5	24302152	42.49	34.06	24276178	96.47	42.38	34.27
ScACS3.1	Static		61	10	20187929	42.48	33.94	20166326	96.42	42.36	34.13
ScACS3.2	SMG		33	9.3	24542608	42.50	34.12	24520924	96.89	42.40	34.25
ScACS4.1	Static		108	10	24586213	42.50	34.10	24563673	96.93	42.39	34.24
ScACS4.2	SMG		156	10	23942119	42.50	34.06	23920514	96.99	42.39	34.21
ScACS5.1	Static		110	9.8	24244159	42.50	34.10	24222229	96.93	42.39	34.24
ScACS5.2	SMG		181	9.9	25369559	42.50	34.06	25347026	96.84	42.39	34.22
ScACS6.1	Static		126	9.7	23496618	42.50	34.12	23473652	96.64	42.40	34.26
ScACS6.2	SMG		175	10	23589464	42.50	34.06	23567332	96.80	42.39	34.21

*Phred Quality Score (−10log₁₀Prob) 30: Base call accuracy 99.9%, 40: Base call accuracy 99.99%.

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