Fig. 1
From: Bulk RNA sequencing analysis of developing human induced pluripotent cell-derived retinal organoids
Overview of the hiPSC-derived culture system and experimental workflow. (A) Schematic diagram of the timeline for organoid differentiation from forced aggregation of hPSCs until the formation of 3D retina cups with photoreceptor outer segments. (B) Live fluorescent micrographs of representative retinal organoids at D47 (i-iv), D90 (v-viii) and D175 (ix-xii) showcasing brightfield (BF), SIX6-eGFP (green), CRX-h2b-mRuby3 (magenta) and Merge at each time point respectively. Scale bar for D47/90/175 is 150 µm. (C) Flowchart highlighting the bulk RNA sequencing workflow including sample collection, RNA extraction/purification, quantification/quality assessment, next generation sequencing and data analysis using open source bioinformatics tools.
