Fig. 3
From: Bulk RNA sequencing analysis of developing human induced pluripotent cell-derived retinal organoids
Overview of sampling and sequence alignment quality. (A) Principal Component Analysis (PCA) plot showing replicates from time points clustered according to their variance in two dimensions. (B) Sample to sample distance heatmap indicating the relative distance between replicates of the same time point and those from different days. (C) HISAT2 paired end (PE) alignment histogram denoting majority of the reads with unique mapping in the human reference genome. (D) Volcano plot representing significantly differentially expressed genes upregulated at D65 versus D25 graphed with respect to -log10(False Discovery Rate) in the y-axis and log2(Fold Change) in the x-axis.
