Table 1 Details of RNA sample pooling and PacBio Iso-seq output statistics.
From: Full-length transcriptome from different life stages of cobia (Rachycentron canadum, Rachycentridae)
Details of RNA samples pooling | |||
|---|---|---|---|
Pool | Description | Life stage | Group |
Pool 1 | Whole Cobia larvae of 5 dph and 29 dph | Larvae | Pool 1 |
Pool 2 | Muscle, kidney, spleen, liver, intestine | Juveniles | Pool 2 |
Pool 3 | Muscle, pyloric caeca, spleen, intestine, kidney, liver | Adults | Pool 3 |
PacBio Iso-seq output statistics | |||
Libraries | Pool 1 | Pool 2 | Pool 3 |
Subreads | 8609435 | 7441673 | 9140164 |
Total bases | 18164983812 | 14795758862 | 18245204020 |
Mean of Longest Subread Length | 2109.9 | 1988.2 | 1996.2 |
Number of circular consensus sequence reads (CCS) | 219158 | 179015 | 222843 |
Mean length of CCSs | 2404.9 | 2188.2 | 2258.9 |
Total bases of CCSs | 527057778 | 391715180 | 503380988 |
Number of full length reads | 8609435 | 7441673 | 9140164 |
Number of full length non chimeric reads | 4517416 | 3900355 | 4791753 |
Number of full length non chimeric reads with poly-A | 4079227 | 3528745 | 4330583 |
Non-redundant isoforms | |||
Total number | 35661 | ||
Total bases | 94193725 | ||
Maximum length | 11372 | ||
Minimum length | 181 | ||
N50 | 2984 | ||
