Fig. 1
Overview of the rat brain Timm-Nissl atlas. (a) Serial coronal rat brain sections, H108, were cryosectioned and stained with the TimmHaug73 modification of Timm’s sulphide silver method (Timm-light or Timm-dark), or Nissl-staining (thionine). (b) Sections from the remaining rat brains were stained following the same procedures as exemplified with H108 above in Fig. 1a, although with individual differences according to sectioning plane and staining. H109 (one hemisphere) was horizontally sectioned and split into subseries and stained with Timm-Haug73 modification of Timm’s sulphide silver method in sequences of different time intervals (30–70 minutes). H200 and H441 were horizontally sectioned and split into subseries of Timm-dark and Nissl, while H201 was sagittally sectioned and stained with Timm-light, Timm-dark and Nissl. (c) The TIFF images of the different subseries of sections, here exemplified with subject H108 (Timm-light, Timm-dark, Nissl), were pre-processed and spatially registered to the Waxholm Space atlas of Sprague Dawley rat brain v4100. Serial section images are shared via the EBRAINS Research Infrastructure, available for interactive inspection in an online viewer tool with atlas overlay.
