Fig. 4
Regional characteristics of the rat brain Timm-Nissl atlas. (a) Olfactory bulb (H108b, Timm-dark coronal series, section #042), (b) magnified view of Timm-dark (above) and b’) Nissl (thionine, below) series visualising different layers of the main olfactory bulb174 (MOB): olfactory nerve layer (MOBnl); glomerular layer (MOBgl); outer plexiform layer (MOBopl); mitral layer (MOBmi); inner plexiform layer (MOBipl); granule cell layer (MOBgr). (c) Coronal view of hippocampal region96,97 (HR) with inset d (H108b, section #237), (d) Magnified view, with inset e, of HR including: cornu Ammonis 1 and 3 (CA1, CA3), dentate gyrus (DG) and mossy fibres (mf, white arrow), (e) Timm-dark (90° rotation, counter-clockwise), magnified view of different layers of CA1176: stratum oriens (or); stratum pyramidale (pyr); stratum radiatum (rad); stratum lacunosum-moleculare (slm); and DG: outer, middle and inner band of the molecular layer (mol); granular layer (gr); polymorph layer/hilus (hil). e’) Nissl (thionine), and e”) Timm-light. (f) Horizontal view of HR with inset g (H200b, Timm-dark sagittal series, section #071), (g) Magnified view, with inset f, of HR including CA1–3, DG, mf (white arrow), subiculum (SUB), presubiculum (PrS), parasubiculum (PaS) and entorhinal cortex (EC), (h) Magnification of border between CA3 and DG, (i) Sagittal view of HR with inset j (H201a Timm-dark sagittal series, section #046), (j) Magnified view, with inset k and l (asterisks indicating a septotemporal gradient), of the HR including CA3, DG, SUB, PrS, PaS and EC, (j’) Adjacent view of Nissl-stained section and magnified view of (k) septal and l) temporal part of CA1 (CA1s, CA1t). (m) Cerebellum and brainstem96 (H108a Timm-light coronal series, section #379) with insets n and p, (n) Magnified view of cerebellar cortical layers including molecular layer (ML), granular layer (GL) and Purkinje cell layer (PL, black arrows). (n’) Adjacent section (Timm-dark) with inset o, magnification of distinct Purkinje cells (black arrows) in adjacent sections stained with (o) Timm-dark, (o’) Timm-light, and (o”) Nissl (thionine). (p) Magnified view of brainstem with inset q and r, (q) magnified view of cells (black arrows) in the mesencephalic trigeminal nucleus43 and (r) a glial cell in the brainstem.
