Table 2 Culture conditions used to obtain a reference transcriptome for P. digitata.
Short reference | Type of medium1 | Medium | Composition | Stress | When the stress is applied |
|---|---|---|---|---|---|
1 | L | V8 - Vegetable Juice | Commercial medium modified: addition of 15 g/L CaCO3, centrifugation and dilution 1:5 of the supernatant with Milli-Q water | — | — |
2 | L | Oat milk | Commercial medium | — | — |
3 | L | RMI glucose vitamins B | RMI2, 10 g/L glucose, vitamins B mix (1 mg/L nicotinic acid, 1 mg/L pyridoxine, 1 mg/L Calcium panthotenate, 1 mg/L Thiamine HCl, 0.1 mg/L Biotin) | — | — |
4 | L | RMI malt extract | RMI2, 10 g/L Malt extract (Difco) | — | — |
5 | L | RMI glucose wheat peptone | RMI2, 10 g/L glucose, 2 g/L wheat peptone HE1 (Organotechnie) | — | — |
6 | L | 1 mM CuS04 | 7 days | ||
7 | L | 200 mM NaCl | 7 days | ||
8 | L | 1 mM H2O2 | 7 days | ||
9 | L | light 16 h photoperiod | all over the experiment | ||
10 | L | cold stress at 4 °C | 15 h before the RNA extraction | ||
11 | L | heat stress at 37 °C | 15 h before the RNA extraction | ||
12 | S | PDA | Potato extract 4 g/L, Dextrose 20 g/L, Agar 15 g/L (Difco) | light 16 h photoperiod | all over the experiment |
