Table 3 A small selection of tunable parameters used to produce the final dataset.

From: Homologous Pairs of Low and High Temperature Originating Proteins Spanning the Known Prokaryotic Universe

Parameter/s

Description

Published value

OGT threshold

Binary threshold to split organisms into mesophiles and thermophiles.

40.0 °C

16s alignment coverage

Minimum alignment coverage (both strands) of 16s rRNA sequence to be considered a taxa pair

98.5%

16s alignment % id

Minimum alignment gap compressed percent identity of 16s rRNA sequence to be considered a taxa pair

81%

protein alignment coverage

Minimum alignment coverage (both strands) of protein sequence to be considered a protein pair

75%

protein alignment E values

Maximum E value of protein alignment to be considered a protein pair

1e-4