Fig. 4

Quality of the purified RNAs, sequencing libraries, and sequencing reads. (a,b) Electropherogram of representative (a) mCARs and (b) cytosolic RNA obtained with the A549 cell. (c,d) Electropherogram of a representative sequencing library from (c) mCARs and (d) cytosolic RNA for the A549 cell. (e) Distribution of quality scores by base pair for a representative FASTQ file from RNA-seq data for the A549 cell. The quality scores are defined as -10log10(P), where P is the probability that a base call is erroneous. In this image, the background colors reflect very good quality calls (green, quality scores: 28-40), calls of reasonable quality (orange, quality scores: 20-28), and calls of poor quality (red, quality scores: 0-20). The blue line in the graph represents the mean quality scores. (f) Distribution of the mean quality score by reads for a representative FASTQ file from RNA-seq data in the A549 cell. The phred scores are also defined as -10log10(P), where P is the probability that a base call is erroneous.