Fig. 3

Phylogenomics based evolutionary history of the genus Clostridium. Maximum likelihood phylogenetic tree (LG4X + F) of the genus Clostridium after adding 270 genomes that were sequenced in this study (highlighted with red circles). Support values are indicated if below 100. Scale bar indicates the average number of substitutions per site. Pairwise average nucleotide identities (ANI) of 92% and above are displayed next to the phylogenetic tree. All major branches leading to species-level clades were fully supported (support value = 100), strain names and within species clade support values are shown in the detailed tree provided as Fig. 1.