Table 1 Methods and outputs for sequencing experiments.
Experiment | Method/Platform | Manufacturer | Insertion size | Output | Coverage |
|---|---|---|---|---|---|
DNA extraction | Magnetic bead method | Invitrogen, Thermo Fisher Scientific, USA | NA | NA | NA |
RNA extraction | TRIzol reagent | Thermo Fisher Scientific, USA | NA | NA | NA |
Short-read seq | NovaSeq 6000; paired-end | Illumina, USA | 350 bp | 68.7 Gb | 115× |
Long-read seq | PromethION | Oxford Nanopore Technologies, UK | N50 = 16.7 Kb | 117.6 Gb | 196× |
Hi-C seq | NovaSeq 6000; paired-end; digested by MboI | Illumina, USA | 350 bp | 178.1 Gb | 297× |
RNA seq | NovaSeq 6000; paired-end | Illumina, USA | 350 bp | 16.3 Gb | NA |
