Fig. 3

Results illustrating the sufficiency of sequencing depth, i.e., the total number of sequencing reads (including fungal and spike reads) obtained for each sample. Panel A shows the distribution of sequencing depth among the samples, with the dashed vertical line corresponding to the value of 10,000 sequence reads, which we recommend using as a threshold for including a sample for analyses. Panel B shows the decrease in the number of species-level OTUs if rarefying all samples to 10,000 sequence reads. Panel C shows rarefaction curves for all samples that included at least 10,000 sequence reads.