Table 1 Description of the HS microscopic system components.

From: Histological Hyperspectral Glioblastoma Dataset (HistologyHSI-GB)

 

Component

Manufacturer

Model

Key Parameter

Microscope

Microscope Model

Olympus, Tokyo, Japan

BX-53

Brightfield microscope

Trinocular Tube

U-TR30IR-1-2

30° inclination and FN22

Eyepiece

WHN10X-H-1-7

Wide field eyepiece FN22

Lenses*

LMPLN-IR

5× and 10×

LCPLN-IR

20× and 50×

Power supply unit

TH4 - 200

12 V 100 W

Lamp House

U-LH100IR-1-7

Transmittance and reflectance

IR Halogen Lamp

Philips, Amsterdam, Netherlands

7724 EVA

400–1800 nm

Stage

Märzhäuser, Wetzlar, Germany

SCAN 130 × 85

3D movement with ± 3 µm resolution

Joystick

M-HID-JS-3

Movement in the 3 axes

Camera Adapter

Olympus, Tokyo, Japan

U-CMAD3-1-7

C-mount

HSI System

HS Camera

HeadWall Photonics, Fitchburg, MA, USA

Hyperspec® VNIR A-Series

Technology

Push-broom scanning

Spectral range

400 to 1000 nm

N° of bands

826 bands

Spectral resolution

2.8 nm

Spatial size

1 × 1004 pixels

FPA Detector

Adimec, Eindhoven, Netherlands

RA1000m

CCD with 7.4 μm pixel pitch

Frame grabber

EPIX, Inc., Buffalo Grove, IL, USA

PIXCI® EL1

PCIe x1 Camera Link Frame Grabber