Table 1 Sources and genome characteristics (base composition, gene size, and gene number) of 90 Buchnera strains used in dataset construction.

1

Muscaphis stroyani

CP034861.1

37.3

36.9

12.8

12.9

74.2

25.7

0.006

0.004

619238

5E + 05

2461

4557

368

77039

565

32

3

1

2

Melanaphis sacchari

CP029161.1

37.2

37.5

12.7

12.6

74.7

25.3

−0.005

−0.004

626137

6E + 05

2554

4562

375

65065

581

33

3

1

3

Schizaphis graminum

CP029205.1

37.2

37.5

12.8

12.5

74.7

25.3

−0.004

−0.01

641689

6E + 05

2472

4562

373

69646

599

32

3

1

4

Rhopalosiphum maidis

CP032759.1

37.2

37.5

12.8

12.5

74.7

25.3

−0.004

−0.01

642929

6E + 05

2467

4553

376

66277

602

32

3

1

5

Rhopalosiphum padi

CP034858.1

37.5

37.2

12.5

12.7

74.7

25.2

0.004

0.008

643941

6E + 05

2475

4558

375

65087

594

32

3

1

6

Aphis nerii

CP034885.1

38.1

37.8

12.1

12.1

75.9

24.2

0.004

0.001

631491

6E + 05

2465

4553

369

61991

589

32

3

1

7

Aphis helianthi

CP034894.1

38.1

37.8

12

12.1

75.9

24.1

0.004

0.001

634211

6E + 05

2471

4548

369

59982

591

32

3

1

8

Aphis aurantii 13

*CP135018

37.6

37.3

12.5

12.6

74.9

25.1

0.004

0.001

629080

6E + 05

2465

4552

369

64084

578

32

3

1

9

Aphis aurantii 14

*CP135021

37.6

37.3

12.5

12.6

74.9

25.1

0.004

0.001

628870

6E + 05

2463

4552

369

62784

575

32

3

1

10

Aphis craccivora

CP043999.1

37.9

37.6

12.2

12.2

75.5

24.4

0.004

0.003

632731

6E + 05

2468

4546

369

66565

579

32

3

1

11

Aphis fabae

CP042427.1

38

37.7

12.1

12.1

75.7

24.2

0.004

0.004

634931

6E + 05

2468

4549

371

61212

589

32

3

1

12

Aphis urticata

CP048744.1

37.5

37.2

12.7

12.7

74.7

25.4

0.004

0

630969

6E + 05

2463

4548

371

69013

591

32

3

1

13

Aphis nasturtii

CP034888.1

37.7

37.4

12.4

12.4

75.1

24.8

0.003

0.001

630331

6E + 05

2379

4548

372

64522

584

31

3

1

14

Aphis gossypii

CP042426.1

37.4

37.2

12.7

12.7

74.6

25.4

0.003

0.002

628324

6E + 05

2464

4550

372

63466

583

32

3

1

15

Aphis glycines

CP009253.1

37.3

37.1

12.8

12.8

74.4

25.6

0.003

−0.001

628164

6E + 05

2458

4550

372

65088

581

32

3

1

16

Pentalonia nigronervosa

CP061275.1

37

36.8

12.9

13.2

73.8

26.1

0.002

0.01

617483

5E + 05

2470

4558

363

116502

522

32

3

1

17

Hyadaphis tataricae

CP034873.1

36.7

36.3

13.5

13.5

73

27

0.006

0.002

633867

6E + 05

2466

4553

370

67308

580

32

3

1

18

Diuraphis noxia

CP013259.1

37.5

37.1

12.7

12.8

74.6

25.5

0.005

0.006

636266

6E + 05

2461

4550

366

69455

586

32

3

1

19

Lipaphis pseudobrassicae

CP034870.1

37.5

37.1

12.4

12.5

74.6

24.9

0.004

0.004

641221

5E + 05

2477

4552

371

92222

578

32

3

1

20

Brevicoryne brassicae

CP034882.1

37.7

37.3

12.5

12.6

75

25.1

0.006

0.003

645850

6E + 05

2469

4549

369

68457

596

32

3

1

21

Brachycaudus cardui

CP034879.1

37.6

37.1

12.6

12.7

74.7

25.3

0.007

0.004

643931

6E + 05

2478

4551

363

65474

591

32

3

1

22

Myzus persicae

CP002699.1

37.5

37.1

12.7

12.8

74.6

25.5

0.006

0.005

643502

6E + 05

2479

4555

363

63744

586

32

3

1

23

Artemisaphis artemisicola

CP034900.1

38

37.5

12.3

12.3

75.5

24.6

0.006

0.001

633406

6E + 05

2478

4550

359

70038

577

32

3

1

24

Hyperomyzus lactucae

CP034876.1

37.2

36.7

13

13.1

73.9

26.1

0.006

0.004

641856

6E + 05

2487

4553

363

66886

591

32

3

1

25

Macrosiphoniella sanborni

CP034864.1

38.1

37.6

12

12.2

75.7

24.2

0.006

0.007

621931

5E + 05

2464

4571

366

82291

544

32

3

1

26

Uroleucon sonchi

CP047588.1

38.1

37.6

12.2

12.2

75.7

24.4

0.006

−0.001

614349

5E + 05

2464

4563

364

80215

539

32

3

1

27

Uroleucon ambrosiae

CP002648.1

38.2

37.7

12

12.1

75.9

24.1

0.007

0.002

615380

5E + 05

2464

4563

368

75953

541

32

3

1

28

Sitobion avenae

CP034855.1

37.2

36.8

12.9

13

74

25.9

0.006

0.005

636177

6E + 05

2479

4552

368

74738

571

32

3

1

29

Sitobion miscanthi

CP084934.1

37.2

36.9

12.9

13.1

74.1

26

0.004

0.008

671355

6E + 05

2479

4549

736

81297

601

32

3

2

30

Acyrthosiphon kondoi

CP002645.1

37.4

36.9

12.8

12.9

74.3

25.7

0.006

0.006

641794

6E + 05

2469

4559

363

73265

581

32

3

1

31

Acyrthosiphon pisum

BA000003.2

37.1

36.6

13.1

13.2

73.7

26.3

0.006

0.006

640681

6E + 05

2477

4549

366

71806

582

32

3

1

32

Acyrthosiphon lactucae

CP034891.1

38

37.5

12.1

12.3

75.5

24.4

0.007

0.008

642335

6E + 05

2478

4548

362

80073

573

32

3

1

33

Microlophium carnosum

CP048747.1

37.5

37

12.7

12.8

74.5

25.5

0.006

0.005

642296

5E + 05

2476

4551

364

89298

569

32

3

1

34

Macrosiphum gaurae

CP034867.1

37.3

36.9

12.8

13

74.2

25.8

0.005

0.008

643561

6E + 05

2474

4550

366

72885

581

32

3

1

35

Macrosiphum euphorbiae

CP033006.1

37.5

37

12.7

12.9

74.5

25.6

0.007

0.007

645334

6E + 05

2473

4550

364

76716

577

32

3

1

36

Formosaphis micheliae 27

*CP135045

38.3

38.2

11.6

11.9

76.5

23.5

0.001

0.012

554708

4E + 05

2536

4586

362

106692

457

33

3

1

37

Formosaphis micheliae 28

*CP135047

38.3

38.2

11.6

11.9

76.5

23.5

0.001

0.011

554353

4E + 05

2631

4587

362

109421

453

34

3

1

38

Baizongia pistaciae

AE016826.1

37.1

37.5

12.7

12.7

74.6

25.4

−0.006

0.001

615980

5E + 05

2474

4574

364

104082

521

32

3

1

39

Melaphis rhois

CP033004.1

36.9

37.1

12.8

12.8

74

25.6

−0.003

0.002

616452

5E + 05

2455

4587

362

103857

542

32

3

1

40

Schlechtendalia chinensis

CP011299.1

37

37.2

12.9

12.9

74.2

25.8

−0.002

0.003

607835

5E + 05

2462

4573

355

89734

547

32

3

1

41

Mindarus japonicus 20

*CP135030

38.4

38.3

11.6

11.7

76.7

23.3

0.002

0.002

541202

5E + 05

2618

4569

363

69261

476

34

3

1

42

Mindarus keteleerifoliae 18

*CP135024

38.1

37.9

12

11.9

76

23.9

0.003

−0.002

542558

5E + 05

2541

4572

366

74558

477

33

3

1

43

Mindarus keteleerifoliae 19

*CP135027

38.1

37.9

12

11.9

76

23.9

0.003

−0.002

542511

5E + 05

2610

4572

366

74688

476

34

3

1

44

Taiwanaphis decaspermi 29

*CP135049

39.7

39.1

10.6

10.6

78.8

21.2

0.008

0.001

453895

4E + 05

2389

4611

363

46053

411

31

3

1

45

Neophyllaphis podocarpi 25

*CP135039

38.7

39

11.2

11.2

77.7

22.4

−0.004

−0.001

559476

5E + 05

2387

4679

362

58020

519

31

3

1

46

Neophyllaphis varicolor 26

*CP135042

38.7

39

11.2

11.1

77.7

22.3

−0.004

−0.003

559524

5E + 05

2390

4670

362

59691

518

31

3

1

47

Anoecia oenotherae

CP033012.1

39.3

38

11.8

11

77.3

22.8

0.016

−0.036

548691

4E + 05

2637

4568

368

114458

444

34

3

1

48

Thelaxes californica

CP034852.1

39.1

38.3

11.4

11.2

77.4

22.6

0.011

−0.009

522699

4E + 05

2385

4698

383

76042

453

31

3

1

49

Kurisakia onigurumii 23

*CP135033

39.9

38.9

10.6

10.6

78.8

21.2

0.012

0

509028

4E + 05

2467

4342

382

62346

448

32

3

1

50

Kurisakia onigurumii 24

*CP135036

39.9

38.9

10.6

10.6

78.8

21.2

0.012

0

509341

4E + 05

2467

4669

383

61962

445

32

3

1

51

Nipponaphis monzeni

AP019379.1

39.2

38.5

11.2

11.2

77.7

22.4

0.009

−0.001

587781

4E + 05

2394

4599

355

145391

445

31

3

1

52

Ceratoglyphina bambusae 01

*CP134982

40.7

39.4

9.8

10.1

80.1

19.9

0.017

0.013

424619

4E + 05

2250

4557

356

45627

388

29

3

1

53

Chaitoregma tattakana 04

*CP134991

39.4

38.2

11.1

11.3

77.6

22.4

0.016

0.009

419464

4E + 05

2327

4636

364

41943

383

30

3

1

54

Astegopteryx bambusae 03

*CP134988

40.8

39.3

9.8

10.1

80.1

19.9

0.019

0.014

411922

4E + 05

2325

4636

366

39456

388

30

3

1

55

Astegopteryx bambusae 02

*CP134985

40.8

39.3

9.8

10.1

80.1

19.9

0.019

0.014

411372

4E + 05

2325

4633

366

38216

384

30

3

1

56

Ceratovacuna japonica

AP026065.1

40.9

39.1

9.9

10.1

80

20

0.022

0.012

414725

4E + 05

2323

4634

356

41805

379

30

3

1

57

Ceratovacuna keduensis 05

*CP134994

41.7

39.9

9.1

9.3

81.6

18.4

0.021

0.015

413541

4E + 05

2327

4637

360

39326

382

30

3

1

58

Pseudoregma panicola 07

*CP135000

41.3

39.6

9.4

9.7

80.9

19.1

0.021

0.014

412898

4E + 05

2324

4629

359

37591

383

30

3

1

59

Pseudoregma panicola 06

*CP134997

41.3

39.6

9.4

9.7

80.9

19.1

0.021

0.014

412562

4E + 05

2324

4629

359

37876

382

30

3

1

60

Stegophylla sp.

Manzano-Marín et al., 2023

38.4

38.6

11.5

11.5

77

23

−0.003

0.001

412953

3E + 05

2473

4565

369

68010

352

32

3

1

61

Phyllaphis fagi

Manzano-Marín et al., 2023

38.6

38.7

11.3

11.4

77.3

22.7

−0.001

0.002

431406

4E + 05

2548

4555

359

63425

370

33

3

1

62

Shivaphis celti 15

*CP134977

37.5

38.2

12.2

12.2

75.7

24.4

−0.009

0

422485

4E + 05

2391

4563

359

36404

388

31

3

1

63

Therioaphis trifolii

CP032996.1

39.8

40

10.1

10.1

79.8

20.2

−0.003

0.001

419293

4E + 05

2489

4551

364

33760

390

32

3

1

64

Sarucallis kahawaluokalani

CP032999.1

37.4

37.8

12.4

12.4

75.2

24.8

−0.004

0.002

428356

4E + 05

2389

4560

368

44242

390

31

3

1

65

Takecallis taiwana 16

*CP134978

37

37.3

12.8

12.9

74.3

25.7

−0.004

0.003

433453

4E + 05

2384

4554

364

43036

396

31

3

1

66

Takecallis taiwana 17

*CP134979

37

37.3

12.8

12.9

74.3

25.7

−0.004

0.003

432979

4E + 05

2384

4554

364

42601

395

31

3

1

67

Greenidea ficicola 11

*CP135012

40.7

40.7

9.3

9.3

81.4

18.6

0

0.004

395344

4E + 05

2234

4566

363

29894

368

29

3

1

68

Mollitrichosiphum nigrofasciatum 12

*CP135015

39

39.3

10.9

10.8

78.3

21.7

−0.005

−0.001

418955

4E + 05

2364

4557

335

50493

374

31

3

1

69

Drepanosiphum platanoidis

Manzano-Marín et al., 2023

41

40.6

9.2

9.2

81.6

18.4

0.004

0

448962

4E + 05

2398

4638

384

67208

379

31

3

1

70

Periphyllus koelreuteriae 21

*CP134980

40.7

41

9.2

9.1

81.7

18.3

−0.004

−0.003

452078

4E + 05

2324

4572

394

54044

408

30

3

1

71

Periphyllus koelreuteriae 22

*CP134981

40.6

41

9.2

9.2

81.6

18.4

−0.005

−0.004

451592

4E + 05

2325

4574

397

56471

406

30

3

1

72

Sipha maydis

CP097205.1

38.8

39.3

10.9

11

78.1

21.9

−0.007

0.003

462390

4E + 05

2401

4583

376

60308

411

31

3

1

73

Periphyllus lyropictus

CP097457.1

40.7

40.4

9.5

9.4

81.1

18.9

0.004

−0.009

456890

4E + 05

2314

4565

377

60459

411

30

3

1

74

Nippolachnus piri 09

*CP135006

39.4

39.6

10.5

10.6

79

21.1

−0.003

0.008

417640

4E + 05

2393

4570

384

47125

377

31

3

1

75

Nippolachnus piri 10

*CP135009

39.4

39.6

10.5

10.6

79

21.1

−0.003

0.008

417660

4E + 05

2393

4570

384

47145

377

31

3

1

76

Tuberolachnus salignus

LN890285.1

39.1

39.3

10.8

10.8

78.4

21.6

−0.003

−0.002

421426

4E + 05

2408

4578

388

47017

377

31

3

1

77

Tuberolachnus salignus 08

*CP135003

39.1

39.3

10.8

10.8

78.4

21.6

−0.003

−0.002

421666

4E + 05

2408

4578

388

47263

382

31

3

1

78

Cinara confinis

LT667503.1

37.9

38.1

11.9

12

76

23.9

−0.002

0.004

443747

4E + 05

2386

4573

387

68133

384

31

3

1

79

Cinara tujafilina

CP001817.1

38.4

38.5

11.5

11.5

76.9

23

−0.002

0.001

444925

4E + 05

2385

4575

384

78487

394

31

3

1

80

Cinara cedri

CP000263.1

40.1

39.8

10

10.1

79.9

20.1

0.003

0.004

416380

4E + 05

2390

4571

366

53361

369

31

3

1

81

Cinara strobi

LR025085.1

38.1

38

11.9

12

76.1

23.9

0.001

0.001

440140

4E + 05

2400

4580

374

67761

372

31

3

1

82

Cinara piceae

LR217739.1

39.2

38.9

11

10.9

78.1

21.9

0.004

−0.004

435017

4E + 05

2392

4595

375

65225

372

31

3

1

83

Cinara curtihirsuta

LR217700.1

39.5

39.3

10.6

10.6

78.8

21.2

0.002

0

433229

4E + 05

2390

4591

377

59409

375

31

3

1

84

Cinara curvipes

LR217710.1

39.5

39.3

10.6

10.6

78.8

21.2

0.002

0.001

433837

4E + 05

2394

4587

374

59504

376

31

3

1

85

Cinara cuneomaculata

LR217695.1

38.3

38.1

11.8

11.8

76.4

23.6

0.003

−0.001

430960

4E + 05

2476

4581

375

66837

368

32

3

1

86

Cinara kochiana kochiana

LR217707.1

38.5

38.2

11.6

11.7

76.7

23.3

0.004

0.003

433740

4E + 05

2386

4585

373

62421

374

31

3

1

87

Cinara laricifoliae

LR217717.1

39

38.7

11.1

11.2

77.7

22.3

0.003

0.002

436713

4E + 05

2394

4586

375

65617

374

31

3

1

88

Cinara pseudotaxifoliae

LT635893.1

38.1

37.8

12.1

12

75.9

24.1

0.004

−0.003

446627

4E + 05

2395

4578

369

70648

378

31

3

1

89

Cinara cf splendens

LR217692.1

38.2

37.8

12

11.9

76

23.9

0.005

−0.005

444797

4E + 05

2393

4575

370

70934

378

31

3

1

90

Cinara splendens

LR217722.1

38.4

38

11.9

11.7

76.4

23.6

0.006

−0.007

445237

4E + 05

2389

4577

371

69992

377

31

3

1