Fig. 4
From: An image-based screen for secreted proteins involved in breast cancer G0 cell cycle arrest
Positive controls for the screen that increase G0 arrest. (a) Representative images of screening controls in MCF7 cells - neutral (PBS), and positive controls for G0 arrest - TGFb1 and Palbociclib. In merged images Hoechst is shown in blue, P-Rb in green, p21 in yellow and EdU staining in red. Scale bar = 50 μM. (b) Frequency distribution of single cell data showing the EdU ratio. Dashed red line represents the cut off between EdU negative (−) and EdU positive cells. (c) Quantification of EdU negative (G0 arrested) cells. Graphs showing the percentage of cells per well which are EdU-. Red line represents mean. Mock = PBS treatment and Vehicle = DMSO. One-way ANOVA was used for statistical significance. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, ns = not significant.
