Fig. 1

Overview of the study design and experimental workflow. RNA-seq data was generated from hematopoietic stem cells (HSCs) isolated from control Mx1-Cre and experimental OsmrΔ/Δ Mx1-Cre mice that had been treated ex vivo for 1 hour with recombinant murine OSM (500 ng/ml) or vehicle control20. An independent cohort of mice was used for technical validation of the genotype and recombination of OsmrΔ/Δ Mx1-Cre as well as validation of reduced OSMRβ expression by Western blotting. Created with BioRender.com.