Fig. 3
Differential gene expression analysis of recombinant OSM-stimulated genes in control Mx1-Cre and OsmrΔ/Δ Mx1-Cre HSCs. (A) Principal component analysis of all biological replicate samples of control Mx1-Cre HSCs treated with OSM (n = 6) or without OSM (n = 6) and OsmrΔ/Δ Mx1-Cre HSCs treated with OSM (n = 3) or without OSM (n = 3)20. (B) Volcano plot showing differential gene expression of OSM-treated vs. vehicle-treated control Mx1-Cre HSCs. Numbers of significantly differentially expressed genes (log2(FC) >2 or <−2, p < 0.05) are indicated. See also Supplementary Table 2. (C) Heatmap of gene expression across all samples of the 322 differentially expressed genes identified in OSM-treated vs. vehicle-treated control Mx1-Cre HSCs. (D) Proportions of the 171 OSM-induced genes in Mx1-Cre HSCs assessed in OSM-treated vs. vehicle-treated OsmrΔ/Δ Mx1-Cre HSCs. Cutoff for significantly induced or repressed genes utilized p < 0.05 criteria. See also Supplementary Table 3. (E) Gene ontology (GO) term enrichment of 151 OSM-induced genes in control Mx1-Cre HSCs that were not induced by OSM in OsmrΔ/Δ Mx1-Cre HSCs.
