Fig. 4
Genotyping and recombination of the Osmrfl locus in tissues from OsmrΔ/Δ Mx1-Cre mice. Agarose gel electrophoresis (2% agarose) of PCR-amplified products. In all gels a 100 bp ladder was used for size estimation located in lane 0. (A) Genotyping PCR for Osmr <tm1.1Nat> from ear punch genomic DNA. Lanes 1–3 are from Mx1-Cre mice, lanes 4–6 are from Osmrfl/fl Mx1-Cre mice, lane 7 is from a wild-type C57BL/6 J mouse and lane 8 is from a positive control Osmrfl/fl confirmed by The Jackson Laboratory’s in-house genotyping service. (B) Genotyping PCR for Cre recombinase from ear punch genomic DNA. Lane 1 is from a positive control, lane 2 is from a wild-type C57BL/6 J mouse, lanes 3–5 are from Mx1-Cre mice and lanes 6–8 are from Osmrfl/fl Mx1-Cre mice. (C) Recombination PCR for the Osmr <tm1.1Nat> allele from indicated tissues. From left, lanes 1–2 are from blood samples from wild-type C57BL/6 J mice. In the remaining images, samples are ordered as lanes 1–3 from Mx1-Cre mice and lanes 4–6 from OsmrΔ/Δ Mx1-Cre mice.
