Fig. 5
Assessment of OSMRβ in tissues from OsmrΔ/Δ Mx1-Cre mice. (A–C) Western blot analysis of cell lysates from (A) liver, (B) kidney, and (C) bone marrow from Mx1-Cre and OsmrΔ/Δ Mx1-Cre mice. Blots were probed with antibodies against OSMRβ (~150 kDa) and β-ACTIN (45 kDa) (n = 3 biological replicates per genotype). (D) Relative protein expression assessed by Western blot imaging using ImageJ. OSMRβ band densities were normalized to β-ACTIN. Dots represent individual mice. *p < 0.05. Significance was calculated using paired t test with p values adjusted for multiple comparisons using the Holm-Sidak method.
