Table 1 Comparison of benchmarks of this assembly with previous grasspea assemblies.
From: A chromosome-scale reference genome of grasspea (Lathyrus sativus)
EIv1 (2020)60 | Rajarammo-han et al.6 | this assembly | |||
---|---|---|---|---|---|
grasspea accession | LS007 | LS007 | Pusa-24 | LS007 | |
Assembly | Sequencing technology | Illumina paired end | PromethION nanopore + Illumina PE | Illumina paired end + PacBio Sequel | PacBio HiFi |
total length | 8.12 Gbp | 6.22 Gbp | 3.81 Gbp | 5.96 Gbp | |
of which N’s | 1920 Mbp | 0 Mbp | 5.44 Mbp | 2.93 Mbp | |
contig N50 | 0.006 Mbp | 0.156 Mbp | 0.078 Mbp | 5.52 Mbp | |
Number of contigs | 669,893 | 162,994 | 80,744 | 8,449 | |
Scaffolding | Scaffolding approach | Illumina long mate pair | HiC (partial) | aligned to Caméor v1a49 | HiC |
scaffold N50 | 0.06 Mbp | 363 Mbp (partial) | 421 Mbp | 700 Mbp | |
total size of top 7 scaffolds | not chromosome-level | 2.51 Gbp | 3.21 Gbp | 5.03 Gbp | |
Merqury | Illumina QV | 41.9 | 15.7 | 18.0 | 42.4 |
Illumina kmer completeness | 92.7 | 77.6 | 48.6 | 90.0 | |
BUSCO | Complete-ness, Viridip-lantae | 86.4% | 89.8% | 98.3% | 99.1% |
Complete-ness, Fabales | 79.9% | 82.6% | 96.0% | 97.4% |