Table 1 Comparison of benchmarks of this assembly with previous grasspea assemblies.

From: A chromosome-scale reference genome of grasspea (Lathyrus sativus)

  

EIv1 (2020)60

Rpb (202060, scaff. 20237)

Rajarammo-han et al.6

this assembly

 

grasspea accession

LS007

LS007

Pusa-24

LS007

Assembly

Sequencing technology

Illumina paired end

PromethION nanopore + Illumina PE

Illumina paired end + PacBio Sequel

PacBio HiFi

total length

8.12 Gbp

6.22 Gbp

3.81 Gbp

5.96 Gbp

of which N’s

1920 Mbp

0 Mbp

5.44 Mbp

2.93 Mbp

contig N50

0.006 Mbp

0.156 Mbp

0.078 Mbp

5.52 Mbp

Number of contigs

669,893

162,994

80,744

8,449

Scaffolding

Scaffolding approach

Illumina long mate pair

HiC (partial)

aligned to Caméor v1a49

HiC

scaffold N50

0.06 Mbp

363 Mbp (partial)

421 Mbp

700 Mbp

total size of top 7 scaffolds

not chromosome-level

2.51 Gbp

3.21 Gbp

5.03 Gbp

Merqury

Illumina QV

41.9

15.7

18.0

42.4

Illumina kmer completeness

92.7

77.6

48.6

90.0

BUSCO

Complete-ness, Viridip-lantae

86.4%

89.8%

98.3%

99.1%

Complete-ness, Fabales

79.9%

82.6%

96.0%

97.4%

  1. BUSCO scores are given for “Complete” (single-copy and duplicated) BUSCOs.