Fig. 4
Spatial evaluation of the identified markers from scRNA-seq data. (a) UMAP visualization depicting epithelial and stromal cell clusters of esophagus and stomach from various developmental stages; cells are colored according to cell type and time point. (b) Clustered dot plot highlighting the relative expression of genes across epithelial and stromal cells of gastroesophageal tissue at developmental stages. Dot size denotes the percentage of cells expressing a gene, while color indicates the scaled mean expression level from high (dark blue) to low (yellow). (c–e) IHC images of mouse esophagus (left panel) and stomach (right panel) tissue sections at embryonic day 19 (E19, upper panel) and adult stages (lower panel) for various markers. (c) CDH1 (green) for epithelia, POSTN (red) for fibroblasts, and ACTA2 (white) for smooth muscle cells. (d, left panel) KRT5 (green) for squamous epithelia, P63 (white) for basal progenitor cells, and LORICRIN (red) for squamous differentiated cells. (d, right panel) CHGA (white) for enteroendocrine cells of the stomach base region, and KRT7 (red) for columnar epithelia. (e) CDH1 (green) for epithelia, GATA6 (red) for columnar epithelial-specific transcription factor, and SOX2 (white) for squamous epithelial-specific transcription factor, along with nuclei staining (blue). Images represent three biological replicates.
