Fig. 5
From: Transcriptome and translatome profiling of Col-0 and grp7grp8 under ABA treatment in Arabidopsis
Reads quality and technical validation of CLIP-seq of Col-0 and pGRP7::GRP7-GFP grp7-1 with ABA or mock treatment. (a) Length distribution of clean reads from CLIP-seq. (b) IGV genome browser tracks show significant CLIP crosslink sites on GRP7 target transcripts. GLYCINE-RICH RNA-BINDING PROTEIN 7 (GRP7), SVB-LIKE (SVB2), ORTHOLOG OF SUGAR BEET HS1 PRO-1 2 (HSPRO2), ASPARTIC PROTEASE IN GUARD CELL 1 (ASPG1) and CCR-LIKE (CCL). (c) RIP-qPCR analysis of CLIP targets that are showed in (b) in pGRP7::GRP7-GFP grp7-1 and Col-0 under ABA or mock treatment. The levels in the GFP-trap precipitate are presented relative to the levels in the input. The values represent the means ± SD of three replicates. Student’s t test: ns, no significant; **, p < 0.01; ****, p < 0.0001. RHIP1 as unbound transcripts serve as negative control.
