Table 1 A list of NCBI accession number.

Library	Accession	Study	Sample name	Biosample accession
plasmid	SRR25109622	SRP446953	SAMN36265897	Plasmid library
host_cell	SRR29155703	SRP509673	SAMN41517651	Host cell library
host_cas9_short	SRR29155702	SRP509673	SAMN41517652	Host Cas9 short term
host_cas9_long	SRR29155699	SRP509673	SAMN41517653	Host Cas9 long term
recombinant_cell	SRR29155698	SRP509673	SAMN41517654	Recombinant cell library
recombinant _cas9_short	SRR29155697	SRP509673	SAMN41517655	Recombinant Cas9 short term
recombinant _cas9_long	SRR29155696	SRP509673	SAMN41517656	Recombinant Cas9 long term
2^nd_cell_sorting_pool_1	SRR29155695	SRP509673	SAMN41517657	Recombinant KO sorted replicate 1
2^nd_cell_sorting_pool_2	SRR29155694	SRP509673	SAMN41517658	Recombinant KO sorted replicate 2
2^nd_cell_sorting_pool_3	SRR29155693	SRP509673	SAMN41517659	Recombinant KO sorted replicate 3
2^nd_KO_sorting_pool_1	SRR29155692	SRP509673	SAMN41517660	Recombinant cell sorted replicate 1
2^nd_KO_sorting_pool_2	SRR29155701	SRP509673	SAMN41517661	Recombinant cell sorted replicate 2
2^nd_KO_sorting_pool_3	SRR29155700	SRP509673	SAMN41517662	Recombinant cell sorted replicate 3

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