Fig. 3

RCPI integration aligns similar cell types from each dataset to form the integrated data atlas. (a) PCA (left) and UMAP (right) representations of the datasets before (left) and after (right) RCPI data integration. Time course dataset (top row) is coloured by time point and signalling perturbation dataset (lower two rows) is coloured by both time point and treatment condition. (b) Distribution of cells from each treatment group in an integrated UMAP. (c) UMAPs showing alignment of select gene expression patterns between datasets of origin, where in each pair of plots cells from the time course dataset are shown on the left and cells from the signalling perturbation dataset on the right. For all plots, light grey “other” points indicate position of cells in the opposite dataset.