Figure 1
From: The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation
LIC2 is required for mitotic progression in HeLa cells. (A) Mitotic index in HeLa cells (y-axis); the % of total cells present in metaphase +/−SD. (3 experiments, n = approximately 500 cells per experiment). (B,C) Rescue of the metaphase arrest by transgenic expression of rat LIC1 (B) and rat LIC2 (C) (3 experiments, n = at least 500 cells per experiment). (D) Fraction of mitotic cells from G that spent >80 minutes in mitosis. p values in all panels are *p < 0.05, **p < 0.01, ***p < 0.001. (E) Still images from movies featuring different siRNA treated HeLa cells expressing H2B-mCherry and GFP-α-tubulin (control, LIC1, LIC2 and LIC 1 + 2 depleted) showing prolonged arrest in mitosis. Time points indicate time after nuclear envelope breakdown. (F) Still images from movies representing effect of dynein inhibitor ciliobrevin (DI) at different concentrations 30 μM (F) and 50 μM (G) with DMSO as control.
