Figure 2
From: The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation
Dynein retains its integrity and mitotic localization upon LIC2 depletion. (A) Immunoprecipitation with IC74 co-precipitates HC irrespective of LIC depletion. In = Input, IP = immunoprecipitation, IB = immunoblot. (B) Representative confocal immunofluorescence images showing localization of IC74 to kinetochores (CREST). (C) Percent HeLa cells showing the proper localization of IC74 to kinetochores (CREST) upon respective siRNA treatment. (3 experiments, n = at least 17 prometaphase cells per experiment). (D) Integrated fluorescence intensity of IC74 staining as a ratio to CREST staining at kinetochores from the cells in C (10 cells per experiment from 3 experiments).
