Figure 3
From: The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation
LIC2 regulates spindle orientation in mitosis. (A) Spindle angle in representative mitotic HeLa cells depicted from 3D reconstructions of confocal z-stacks, after respective siRNA treatment. (B) Fraction of metaphase cells with a spindle angle of >20 degrees. Error bars = +/− SEM from 3 independent experiments, at least 20 metaphase cells per experiment. (C) Still images from time-lapse movies of HeLa cells exiting mitosis showing uneven post-mitotic flattening upon LIC2 depletion at various time points after rounding up; arrows indicate daughter cells. (D) Percent mitotic cells showing uneven flattening (3 experiments, n = minimum 10 mitotic cells per experiment). Error bars are mean +/− SD from 3 independent experiments. (E) Rescue of the spindle orientation defects by transgenic expression of rat LIC2. Y-axis represents the spindle angle with respect to the substratum (at least 30 cells over 3 experiments, Error bars are mean +/− SEM, p values in all panels are *p < 0.05, **p < 0.01, ***p < 0.001).
