Figure 1 | Scientific Reports

Figure 1

From: A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags

Figure 1

Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP- and CLIP-tag fused to the protein of interest bind benzylguanine- or benzylcytosine-substrates, respectively. There are various cell-permeable fluorescent substrates and a non-fluorescent substrate available. (b) For labeling of the whole fusion protein pool cells are incubated with a fluorescent substrate, washed and fixed/imaged. (c) For age-dependent labelings cells are first incubated with the non-fluorescent blocker followed by labeling of the newly-synthesized fusion protein with a fluorescent substrate. To omit continuous labeling with the first substrate additional blocking/labeling steps can be added.

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