Figure 2 | Scientific Reports

Figure 2

From: A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags

Figure 2

Age-dependent labeling of insulin SGs and super resolution CLEM. (a) SIM image of Tokuyasu section of SOFIA mouse beta cells labeled according to dual-labeling protocol with 505+ (green), 505+/TMR+ double labeled and TMR+ (magenta) SGs. (b) Overlay of SIM image with corresponding TEM image. (c) Detail from (b) 505+ and TMR+ SGs are precisely corresponding to their TEM counterparts. (d) TEM detail, arrowheads point to correlated 505+ SGs, Scale bars: 1 µm.

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