Figure 5 | Scientific Reports

Figure 5

From: A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags

Figure 5

Super resolution CLEM in Epon sections. (a) SIM image of Epon section of SOFIA mouse beta cells labeled with 505-Star (green) and TMR-Star (magenta) fixed by HPF followed by FS. As fiducial markers fluorescent beads (blue) were added to the section prior to imaging. Punctate SGs can be unequivocally distinguished by SIM. (b) CLEM image of boxed area in (a). (c) CLEM detail of boxed area in (c) showing perfect overlay of 505+ and TMR+ SGs. Scale bars: (a) 10 µm. (b+c) 1 µm.

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