Figure 7 | Scientific Reports

Figure 7

From: A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags

Figure 7

CLEM in Epon after HPF + FS and after chemical fixation. (a–d) SNAP-CCF. (a) Wide-field FLM image of Epon section of INS-1 cells with TMR+ ICA512-CCF-SNAP showing cytosolic and nuclear labeling. (b) CLEM image corresponding to the boxed area in (a) allowing to discriminate labeled from unlabeled cells. (c) CLEM detail corresponding to the boxed area in (c). (d) TEM detail showing nucleus, cell membrane and insulin SGs. (e–h) lifeAct-CLIP. (e) SIM image of an Epon section of INS-1 cells with TMR+ lifeAct-CLIP. (f) CLEM image corresponding to the boxed area in (e) with microvilli correlated to the fluorescent signal. (g) Single slice of tomogram corresponding to (f) with microvilli in boxed area. (h) Detail of boxed area in (f and g). Scale bars: (a) 10 µm. (e) 5 µm. (b–d) + (f–h) 1 µm.

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