Figure 4
Rv2170 modifies ICDH1 using CoA substrates from TCA cycle and methylcitrate cycle. (A) The protein acetyltransferase Rv2170 modifies ICDH1 by using CoA substrates, acetyl-CoA, succinyl-CoA, and propionyl-CoA. (B–D) ICDH1 was incubated with [1,2-14C] acetyl-CoA (B), [1-14C] propionyl-CoA (C), or [1,4-14C]succinyl-CoA (D) in the presence or absence of Rv2170. Proteins were resolved by SDS-PAGE and visualized with Coomassie Blue. Acetylation of proteins was detected by phosphor image.
