Figure 7
PDX up-regulated the expression of M2 markers in RAW264.7 cells. RAW264.7 cells were stimulated by vehicle (0.03% ethanol) or different concentrations of PDX (10 nM, 100 nM or 1000 nM) for 24 h in the absence or presence of GW9662 (1 µM). The expression of Arg1, Ym1 and PPAR-γ was analyzed by Western blot (A,B). And the effect of PDX (1000 nM) treatment was blocked by a PPAR-γ antagonist GW9662 (C,D). The effect of siRNA against PPAR-γ on the expression of PPAR-γ, Ym1 and Arg1 was also assessed by Western blot (E,F). Quantification for protein expression was normalized by GAPDH. Data represented the mean ± SEM from three independent experiments. *P < 0.05, # P < 0.01.
