Figure 8

PDX induced M2 polarization in vitro. RAW264.7 cells were challenged with vehicle or PDX (1000 nM) in the absence or presence of GW9662 (1 µM) for 24 hours. Macrophage marker F4/80 (pan marker, green) and M2 phenotype makers (Arg1 and Ym1) (red) were measured by immunofluorescence staining (A,B). Nuclei were stained with DAPI (blue).